Dr. Indira Hinduja

Research Projects Completed:

• Development of Reproductive Technologies- IVF-ET, GIFT,ZIFT,ICSI,PGD.
• Established for the first time in India the technique of In Vitro Fertilization and Embryo Transfer as a Collaborative efforts between King Edwards Memorial Hospital and Institute of Research in Reproduction (ICMR).
• These efforts resulted in a birth of India's first scientifically documented IVF baby (Test-tube baby) on 6th August 1986.
• Gamete Intrafallopian Transfer (GIFT) technique was developed successfully and India's GIFT baby was born on 4th January 1988.
• Oocyte donation programme in cases of premature ovarian failure. First baby was born on 24th Janaury, 1991.
• Cytogenetic study in couple having unexplained infertility, male infertility-Bad obstetric history.
• Human chorionic villous sampling in high risk pregnancy with history of genetic disorders in past or family history of congenital anomalies.
• Study of chromosomal anomalies in couples belonging to cases having H/O repeated fetal losses or susceptible to fetal loss. Cytogenital study was supported by Research Society of KEM Hospital.
• Induction of Ovulation.
  • Administration of Clomiphene Citrates, human menopausal gonadotropin, pure follicular stimulation hormone and human chorionic gonadotropins in different combination and dose regime.
  • Effect of oral contraceptives prior to ovarian stimulation its effect on follicular response to stimulation protocol.
  • Influence of Gonadotropin Releasing Hormone on stimulation of multiple Follicles.
  • Varying time interval of gonadotropin administration and its effect on oocyte maturation and fertilization in vitro.
• Human Oocyte.
  • Ultrastructure of immature, mature, atretic, normally fertilized and abnormally fertilized oocyte.
  • Correlation between steroid hormones and protein contents of follicular fluid and maturation of oocyte.
  • Protein synthesis by granulosa cells surrounding oocyte and fertilizing ability of oocyte.
  • Constituents of oocyte environment, study of the follicular fluid for the presence of gonadotropin, steroid hormones, glycosaminoglycans, total protein and glucose.
  • Inhibin in follicular fluid and its correlation with maturation, fertilizing ability of oocyte.
  • Intraovarian factors contributing follicular maturation, fertilization and atresia.
  • Effect of injection of purified human follicular fluid on mice follicles.
• Male Infertility
  • Ultrastructure of sperm in infertile male
  • Decondensation of sperm in vitro in infertile male
  • Study of fertilising potential of sperm. Hypoosmotic test, acrydine orange test, zona free hamster egg penetration with human capacited sperms.
• Cytogenetic Study of Oligospermia
• Unexplained Infertility- Immunological factors responsible of infertility by reducing fertilization potential of gametes.
• Intra Uterine Insemination of washed spermatozoa and its outcome in terms of pregnancy.
• Early Pregnancy Factor.
  • Presence of early pregnancy factor in culture fluid and serum as a marker for early embryo implantation.
  • Early diagnosis and prognosis of choriocarcinoma and vesicular mole by estimating early pregnancy factor in serum.
• Vitamin Carrier Protein.
  • Riboflavin-carrier protein (RCP) in follicular fluid and its correlation with oocyte maturation.
  • Presence RCP in fallopian tube in different phases of menstrual cycle.
• Testing of efficacy of ZK 98.299 as a synthetic progesterone antagonist.
• Micromanipulation of Gametes.
  • Microfertilization by injecting a sperm in perivitalline space of oocyte ooplasm.
  • Intracytoplasmic sperm injection.
  • Microbiopsy of mice embryo.
  • Prevention of sex linked diseases.
• Inhibin in endometrium during various phases of the ovarian cycle.
• Hypertrophy of Pituitary during different phases of menstrual cycle.
• Pituitary size and its correlation with follicular size and levels of gonadotropins and steroid hormones.
• Molecular studies on defects in human sperm. Jaslok Hospital & Research Centre.
• Genetic evaluation of male infertility (Y deletion). Jaslok Hospital & Research Centre

Ongoing Research Projects:

• Microdeletion of Y chromosomes in males (collaboration with TIFR)
• Sperm function tests and fertilization in vitro.
• Role of Centrosome in fertilizing potential of the sperm. (collaboration with IRR, ICMR).
• Receptive genes in endometrium useful for implantation. (collaboration with IRR, ICMR).
• To establish human embryonic stem (HES) cell lines without mouse embryonic fibroblast feeder layer and to identify factors secreted by MEF layer that prevents differentiation of HES cells in-vitro. National Institute for Research and Reproduction.
• Purification and characterization of human sperm adhesion molecular identified by polyclonal /monoclonal antibodies and their role in infertility.
• Factors regulating endometrial receptivity in nonhuman primate

List of Publications:

• Hinduja, I. N., Asgaokar V.S. (1969) Single dose of Chloramphenicol in treatment of Enteric Fever. Current Medical Journal., 4, 102-105.
• Hinduja, I. N., Shah S.K. (1969) Ovarian Tomours. Current Medical Journal., 4, 311-314.
• Hinduja, I. N., Thakur S.S. (1971) Septic Abortion (Criminal Abortions). Indian Journal of Obstet. and Gynaecol., 21, 251.
• Hinduja, I. N., Joglekar, S.J. (1974) The comparative study of Coagulation Mechanism in Mid-trimenster Abortions Induced by Prostaglandins and Hypertonic Saline. Indian Journal of Obstet. & Gynaec. 24, 432.
• Hinduja, I. N., Mehta, A.P. (1976) Anaerobic Infections in Obstetrics and Gynaecology. Proceeding of the Third All India Congress of Medical Microbiologists, pp. 323.
  Click here to read the complete list

Contribution to the Books

• Critical appraised of tubal surgery in infertility.' Zaveri K. L., Hinduja I. N. In: 'Progress in infertility & transvaginal sonography' (eds.S.Desai & G. Allahbadia) CBS Publishers & Distributors N. Delhi. pp.243-248 (1996)
• Intrauterine & intratubal insemination. Zaveri K. L., Hinduja I. N. In: ''Infertility & Transvaginal sonography current concepts.": (eds. S. Desai & G. Allahbadia). Jaypee Brothers. pp. 170-176 (1995).
• The role of transvaginal colour Doppler sonography in Assisted Reproductive Techniques. Zaveri K. L., Hinduja I. N.," Transvaginal sonography in infertility' (ed. G. Allahbadia) pp. 48-52 (1998) Rotunda Medical Technologies Pvt. Ltd. Role of transvaginal Sonography in IVF.
• Transvaginal Sonography in Assisted Reproductive Techniques (ART) "Transvaginal sonography in infertility' Hinduja I. N., Zaveri K.L. (ed. G. Allahbadia) pp. 491-495. Rotunda Medical Technologie Pvt.Ltd. Role of transvaginal Sonography in IVF.

Edited Book

Editor of The Book "Assisted Reproductive Technologies, Current Methods and Future Directions" by Oxford University Press, New Delhi, 2001.
On editorial Board "The National Medical Journal Of India".

Conferences and Seminars

• Anaerobic infections and their management in Obstetrics and Gynaecology. Hinduja, I.N, Seminar on Non Spore Bearing infections in Obstetrics and Gynaecology-held at Ahmedabad, organised by Medical Practitioners of Ahmedababd in 1980.
• Anaerobic in Obstetrics and Gynaecology VIIth All Gujarat Obstetrics and Gynaecology Congress, Hinduja I.N, Mehta AP, 1980.
• Role of Anaerobes in infection of the female genital tract. Hinduja, I.N., Mehta AP. 3rd All India Annual Congress of Medical Microbiologists, 24th and 25th November, 1980.
• 4. Place of Laparoscopic Sterilization via fallope rings in Camps. Hinduja I.N., Purandare VN. 1st National Congress on Gynaecological Endoscopy. 14th, 15th ,16th March 1979.

Invited Lectures and Seminars

• In vitro fertilization and embryo transfer and gamete intra fallopian transfer. Annual Meeting of Obstetrics and Gynaecology Society of Trivandrum, Guest Lecture, 26th June, 1987.
• Jaipur University guest lecture in IVF-ET and other assisted reproductive technologies 30th December, 1987.
• In vitro fertilization and embryo transfer following different ovarian stimulation protocols. 1st National Meeting of Indian Society for the study of Reproduction and Fertility 4th November, 1988.
• Role of LHRH Agonists in Obstetrics in Obstetrics and Gynaecology. Recent Advances in Obstetrics and Gynaecology. An Update. November, 1989.
• Role of assisted reproductive technologies in management of infertility. Obstetrics and Gynaecology Society of Ahmedabad. Guest Lecture 17th November, 1989.
• Embryo: Diagnosis and treatment. 18th Annual Conference of the Bombay Obstetrics and Gynaecology Society. 7th April 1990.